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关于我们上海快3 > 产品中心 > 细胞生物学 > 荧光探针与细胞染色 > FS1312PKH26 红色荧光细胞链接试剂盒
  • PKH26 红色荧光细胞链接试剂盒
  • 型号:FS1312
  • 报价:
  • 地区:上海市
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  • PKH26 红色荧光细胞链接试剂盒 PKH26是一种砖利的膜标记探针,结构上带有-长脂肪族尾巴,能稳定插入细胞膜脂质区域。PKH26荧光处于黄橙色光谱区间(见图1) , *大激发波长为551nm,*大发射波长是567nm ,与罗丹明或PE检测系统兼容。
  • 025-58690567
产品详细介绍

PKH26 Red Fluorescent Cell Linker Mini Kit

PKH26红色荧光细胞链接试剂盒

PKH26 红色荧光细胞链接试剂盒PKH26 红色荧光细胞链接试剂盒

产品简介
    PKH26是一种砖利的膜标记探针,结构上带有-长脂肪族尾巴,能稳定插入细胞膜脂质区域。PKH26荧光处于黄橙色光谱区间(见图1) , *大激发波长为551nm,*大发射波长是567nm ,与罗丹明或PE检测系统兼容。也可能用标准荧光素( Fluorescein)激发滤片,但荧光强度可能会有些降低。PKH26具 *长的体内半衰期,超过100天。

BENCHANPINPKH26YINGGUANGXIBAOLIANJIESHIJIHECAIYONGYONGYOUZHUANLIDEMOBIAOJIJISHU,ZHUANLIMOBIAOJIJISHU,WENDINGDEYUXIBAOMOZHIZHIQUJIEHEBINGFACHUHONGSEYINGGUANG,RANSEFANGSHIYILAIYUXIBAOYUMODELEIXING,ZHUYAOYONGYUXIBAOTIWAIBIAOJI、TIWAIXIBAOZENGZHIYANJIUJITINEIWAIXIBAOSHISHIZONGYANJIU。

 产品组成

产品名称

                                             编号

PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane LabelingPKH26红色荧光细胞链接试剂盒

ZUFEN

FS1312-100uL

FS1312-200uL

FS1312-1mL

上海快3SHIJIA:PKH26RANLIAO*1×10-3M(RONGYUYICHUN)

1x100uL

2x100uL

10*100uL

SHIJIB:XISHIYEC

10Ml

30Ml

120ML

自备材料

上海快31、CHONGFENFENSANDEDANGEXIBAOXUANYE

2、HANXUEQINGDEZUZHIPEIYANGJI(WANQUANPEIYANGJI)

上海快33、WUCa2+,Mg2+HEXUEQINGDEPEIYANGJIHUOZHEHUANCHONGYAN(RUDulbecco’s PBSHUOHank’s BSS)

4、XUEQING,BAIDANBAIHUOQITAZUZHIJIANRONGXINGDANBAI

上海快35、LIXINGUAN(4-15mL)

6、WENKONGLIXINJI(1,000×g)

上海快37、YINGGUANGFENXISHEBEI(YINGGUANGDUBANJI,YINGGUANGHUOGONGJUJIAOXIANWEIJING,LIUSHIXIBAOYI)

8、CENGLIUZHAO

9、XUEQIUJISHUBANHUOXIBAOJISHUQI

10、ZAIBOPIANHEGAIBOPIAN

操作方法(下列过程*终体积为2mL,含2uM PKH26,以及1×107cells/mL细胞浓度)

(以下过程均在室温下进行(20-25℃)
1. 将2×107个细胞于离心管中,用不含血清培养基洗一遍。

【ZHUYI】XUEQINGDANBAIHEZHIZHIHUIYURANLIAOJIEHE,JIANGDIYUXIBAOMOJIEHEDERANLIAONONGDU。*HAOZAIYONGXISHIYECZHONGXUANXIBAORANSEQIAN(DISIBU)YONGWUXUEQINGPEIYANGJIHUOHUANCHONGYEXIXIBAOYICI(**BU)。

2. 400×gLIXIN5FENZHONG。【ZHUYI】PKH26RANLIAOBUNENGZHIJIEJIADAOLIXINCHENDIANZHONG,ZHEIYANGHUIZAOCHENGXIBAORANSEBUJUNYIHEXIBAOHUOLIJIANGDI。

3. LIXINHOU,XIAOXINXIQISHANGCENGQINGYE,SHENGYUSHANGCENGXI BAOYETIJIBUCHAOGUO25μL。

【ZHUYI】WEIDEDAOKEZHONGFUDESHIYANJIEGUO,ZAIYONGXISHIYECZHONGXUANSHI,JIANSHAOCANLIUPEIYANGJIHUOHUANCHONGYETIJIFEICHANGZHONGYAO。

4.JIARU1mLXISHIYECYONGYIYEGUANQINGQINGCHUIDAHUNYUN,ZHIBEI  2×XIBAOXUANYE。BUYAOYONGZHENDANGQIZHENDANG,BUYAORANG XIBAOZAIXISHIYECZHONGBAOCUNTAIZHANGSHIJIAN。

【ZHUYI】SHENGLIYAN(physiologic salts)DECUNZAIHUISHIDERANLIAOJIETUANBINGDAFUJIANGDIRANSEXIAOLV。YINCI,XUQUEBAORANSESHIXIBAOXUANFUYUXISHIYECZHONG,BUHANPEIYANGJIHUOHUANCHONGYAN。

5.LINRANSEZHIQIAN,JIANG4μL PKH26YICHUNRONGYEJIA RU1mLXISHIYECZHONG,CHONGFENHUNYUN,PEIZHIDE  2×RANSEYE(4×10-6M)。

【ZHUYI①】:WEIJIANSHAOYICHUNDUIXIBAOHUOLVDEYINGXIANG,BUZHOU5JIARUDERANLIAOSHIDEBUZHOU6ZHONGYICHUN*ZHONGNONGDUBUNENGCHAOGUO1-2%。

上海快3【ZHUYI②】:RUGUOSUOXURANLIAO*ZHONGNONGDU<2×10-6M,XUYONG100%YICHUNJIANGPKH26XISHIYULINGYIDANDUDERONGQIZHONG,YIQUEBAOSHIYANJIEGUODEKEZHONGFUXING。

6. KUAISUJIANG1mL 2×XIBAOXUANYE(BUZHOU4)JIARU  1mL 2×RANSEYE(BUZHOU5)ZHONG,LIJIYONGYIYE GUANHUNYUN。*ZHONGXIBAONONGDUWEI1×107/mL,PKH26NONGDUWEI2×10-6M。

上海快3【ZHUYI】:YOUYURANSESHUNJIANWANCHENG,KUAISUJIANGXIBAOYURANLIAOHUNYUNDUIDEDAOMINGLIANG、JUNYUNHEKEZHONGFUDEBIAOJIJIEGUOFEICHANGZHONGYAO。XIALIECUOSHIYOUZHUYUDEDAOJIAOYOUDEJIEGUO:

a.BUYAOJIANGPKH26RANLIAOZHIJIEJIARUHAN2×XIBAODEXISHIYECZHONG。

上海快3b.JIANG2×XIBAOXUANYE(BUZHOU4)YU2×RANSEYE(BUZHOU5)DENGTIJIHUNHE。

c.DIAOZHENG2×XIBAOHE2×RANLIAODENONGDUBIMIANRANSE TIJITAIXIAO(<100μL)HUOTAIDA(>5mL)。

d.YONGDIANDONGYIYEQIKUAISUJIANGXIBAOHERANLIAOHUNYUN。 XUEQINGYIYEGUANHUNYUNSUDUTAIMANERSHIDERANSEBU JUNYUN。RackingHEXUANWOZHENDANGHUNYUNTONGYANGHUNYUN JIAOMAN,RANSEJUNYIXINGJIAOCHA。

上海快3e.FENPEITIJIJINLIANGZHUNQUE,YIBAOZHENGYANGPINYUYANGPIN ZHIJIAN,SHIYANYUSHIYANZHIJIANDEKEZHONGFUXING。

7.HUNYUNHOUDERANSEDEXIBAOFUYU1-5 min。YOUYU RANSESUDUJIAOKUAI,YANZHANGFUYUSHIJIANDUISHIYANMEIYOUBANGZHU。

【ZHUYI】:RANGXIBAOZAIRANSEYEZHONGTINGLIUJINLIANGDUANDESHIJIAN,TONGSHIBAOZHENGDEDAOLIXIANGDERANSEQIANGDU。YINWEIXISHIYECQUESHAOSHENGLIXINGYAN,GUOZHANGSHIJIANDEBAOLUZAIXISHIYECZHONGHUIZAOCHENGMOUXIEXIBAOHUOLIJIANGDI。RUGUOBUNENGQUEDINGQIYINGXIANGCHENGDU,KEZENGJIAJINZUOXISHIDEDUIZHAOZUHEZHIYONGYICHUNERBUYONGRANLIAORANSEDEDUIZHAOZU。

8. JIARUDENGTIJIDEXUEQING(2mL)HUOQITAHESHIDEDANBAIRONGYE(RU1% BSA)ZHONGZHIFANYING,FUYU1minYIJIEHEDUOYUDERANLIAO。

【ZHUYI①】:XUEQING(HUODENGXIAODEDANBAINONGDU)WEI*YOUDEZHONGZHIYE。RUGUOYONGWANQUANPEIYANGJITIDAI,TIANJIATIJIWEI10mL。

上海快3【ZHUYI②】:BUYAOTONGGUOJIAXISHIYECHUOLIXINLAIZHONGZHIFANYING。

上海快3【ZHUYI③】:BUYAOYONGWUXUEQINGPEIYANGJIHUOHUANCHONGYAN,TAMENHUISHIRANLIAOCHANSHENGJUJI。RANLIAOJUJISHIQINGXIGUOCHENGZHONGWUFAXIJING,SHIDEFENXIGUOCHENGZHONGRENGCUNZAIWEIBIAOJIDEXIBAO。

上海快39.JIANGXIBAOZAI20-25℃TIAOJIANXIA400×gLIXIN10min,XIAOXINXIQISHANGQING。YONG10mLWANQUANPEIYANGJIZHONGXUAN,JIANGZHONGXUANYEZHUANYIZHILINGYIXINDEWUJUNLI XINGUANZHONG,20-25℃TIAOJIANXIA400×gLIXIN5 min。YONG10mLWANQUANPEIYANGJIZAIQINGXILIANGBIANYICHUQUMEI JIEHEDERANLIAO。

上海快3【ZHUYI①】:JIANGZHONGXUANYEZHUANYIZHIXINLIXINGUANZHONG,JIANSHAOLELIXINGUANBICANLIUDERANLIAODUIXIDIXIAOLVDEYINGXIANG;

【ZHUYI②】:BUYAOYONGXISHIYECQINGXI。

10. *HOUYIBUQINGXIHOU,YONG10mLWANQUANPEIYANGJIZHONGXUANXIBAOPINGGUXIBAOHUISHOULV,XIBAOHUOLVHEYING GUANGQIANGDU(TU2。LIXINZHONGXUANXIBAOZHISUOXUHUOXI BAONONGDU。

上海快3【ZHUYI①】:RANSEHOUDEXIBAOKEYIYONGZHONGXINGJIAQUANGUDING,BIGUANGTIAOJIANXIA,YINGGUANGQIANGDUZHISHAO3ZHOUNEIBAOCHIWENDING。

上海快3【ZHUYI②】:RANSEYINGGUANGQIANGDUYIBANWEIBEIJINGDE100-1000BEI。SUIRANRANSEDECVZHIYUXIBAOZHONGLEIYOUGUAN,DANYINGGUANGFENBUYINGGAIJINLIANGJUNYUNDUICHENG。

上海快311、MC-38 TILXIBAOYONGSHANGSHUZHIDINGPKH26NONGDURANSE,*ZHONGXIBAONONGDUWEI1×107/ml。HUOLI(▲)YOUFITCRANSECEDING,PINGJUNYINGGUANGQIANGDU(●)YONGLIUSHIXIBAOYIJIANCE。YONG20μM PKH26BIAOJIHOU,KANGZHONGLIUTILTEYIXINGHEXIAONENGMEIYOUGAIBIAN。

 运输和保存方法

上海快3BINGDAIYUNSHU。2-8℃BAOCUN,YOUXIAOQI1NIAN。PKH26YICHUNRONGYELENGZANGBAOCUN。BAOZHENGRANLIAOYICHUNRONGYEPINGGAIGAIJINJIANSHAOZHENGFAYIFANGZHIRANLIAONONGDUSHENGGAO。

 注意事项

1、QINZHIXINGRANLIAOJIEHEDAOXIBAOMOSHANGWANCHENGBIAOJI。RANSEQIANGDUSHIRANLIAONONGDUHEXIBAONONGDUDEHANSHU,YUSHENTOUXINGWUGUAN。YINCI,BAOZHENGRANLIAOTIANJIALIANGBUGUOLIANGFEICHANGGUANJIAN。GUOBIAOJIDEXIBAOJIANGHUIDAOZHIXIBAOMOWANZHENGXINGQUESHIHUOJIANGDIXIBAOHUOXING。

2、BENPINKEYONGYUTINEITIWAIXIBAODEBIAOJI,BAOKUOGANXIBAO、**XIBAO、DANHEXIBAO、NEIPIXIBAO、SHENJINGXIBAOHUOZHERENHEQITAXIBAO。TINEIXIBAODEBIAOJIGUOCHENGXUYIDINGDEGAIJIN,RUXUEXIAOBANDERANSE,HUOZHEXUANZEXINGBIAOJITUNSHIXIBAO。

3、BENPINRANSEGUOCHENGZHONGXIBAONONGDUHERANLIAONONGDUDAIBIAOCAOZUODEQISHINONGDU。GAINONGDUBEIZHENGMINGSHIYONGYUDUOZHONGXIBAO。SHIYONGZHEXUTONGGUOPINGGURANSEHOUXIBAOHUOLV(RU,PIRANSE)、YINGGUANGQIANGDU、RANSEJUNYUNDUJISHIFOUDUISUOYANJIUXIBAOGONGNENGYOUYINGXIANGDENG,GENJUSHIYANMUDE,QUEDING*YOUDERANLIAONONGDUHEXIBAONONGDU。

4、SUIRANTIEBIXIBAOYEKEYIRANSE,DANDANGEXUANFUXIBAORANSEJUNYIXINGGENGJIA。YINCIYONGDANBAIMEI(trypsin/EDTA)JIANGTIEBIXIBAOXIAOHUACHENGDANGEXUANFUXIBAOHOUZAIRANSEXIAOGUOGENGJIA。

5、PKH26RANSEGUOCHENGZHONG,BUNENGCUNZAIDIEDANHUAWUHUODAIXIEDUXINGWU。

上海快36、YINGGUANGRANLIAOJUNCUNZAICUIMIEWENTI,QINGJINLIANGZHUYIBIGUANG,YIJIANHUANYINGGUANGCUIMIE。

上海快37、GAIRANLIAOBIGUANGBAOCUN,SHIYONGQIANGUANCHASHIFOUYOUJIEJING。RUCHUXIANJIEJING,SHIWENFANGZHI30min,RANHOUCHAOSHENGHUOZHENDANGZHIZHIRONGJIE。

8、XISHIYECKESHIWENHUOLENGZANGBAOCUN。RUGUOLENGZANGBAOCUN,SHIYONGQIANFUWENZHISHIWEN。XISHIYECWEIWUJUNRONGYE,BUHANRENHEFANGFUJIHE***,QIXUBAOCHIWUJUN。BUYAOJIANGRANLIAOCHUCUNYUXISHIYECZHONG。RONGYUXISHIYECDEGONGZUOYEXUXIANPEIXIANYONG,QIEPEIHAOHOUXULIJISHIYONG。

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