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关于我们上海快3 > 产品中心 > 细胞生物学 > 荧光探针与细胞染色 > FS1169JC-10线粒体膜电位检测试剂盒 荧光探针染色
  • JC-10线粒体膜电位检测试剂盒 荧光探针染色
  • 型号:FS1169
  • 报价:1600
  • 地区:上海市
  • 0
  • JC-10 Mitochondrial Membrane Potential Assay Kit JC-10线粒体膜电位检测试剂盒;;Rhodamine 123 罗丹明123;JC-1;JC-10;CCCP;JC-10线粒体膜电位检测试剂盒 荧光探针染色
  • 025-58690567
产品详细介绍

JC-10 Mitochondrial Membrane Potential Assay Kit JC-10线粒体膜电位检测试剂盒

产品信息

产品货号产品名称规格价格(元)
FS1169-100TJC-10 Mitochondrial Membrane Potential Assay Kit  JC-10线粒体膜电位检测试剂盒100T1600.00 

JC-10线粒体膜电位检测试剂盒 荧光探针染色 JC-10线粒体膜电位检测试剂盒 荧光探针染色​   

产品描述

XIANLITIMODIANWEIYINGGUANGTANZHENJC-10SHIJC-1DESHENGJICHANPIN,TONGYANGKEYONGYUJIANCEXIANLITIMODIANWEIDEBIANHUA。YINJC-1SUIRANZAIXUDUOSHIYANZHONGBEIGUANGFANYINGYONG,DANSHIQISHUIRONGXINGHENCHA,JISHIZAI1 μMNONGDUDETIAOJIANXIA,JC-1YEHUIZAISHUIDEHUANCHONGYEZHONGXICHU。ERJC-10JUYOUGENGHAODESHUIRONGXING,KEYIZAIMOUXIEXUYAOGAONONGDURANLIAODESHIYANZHONGTIDAIJC-1。SUIRANZAIYIXIEXIBAOXIZHONGJC-10YOUZHEBIJC-1GENGHAODEBIAOXIAN,BUGUO,JC-10DEXINGNENGBIAOXIANJIJUXIBAOYILAIXINGTEZHENG。

ZHENGCHANGXIBAONEI,JC-10XUANZEXINGJUJIZAIXIANLITIJIZHIZHONGXINGCHENGKENIDEHONGSEYINGGUANGJUHEWU(Ex=540 nm, Em=590 nm);BUJIANKANGDEXIANLITIYOUYUMODIANWEIDEXIAJIANGHUOSANGSHI,JC-10YOUDUOJUTIZHUANBIANWEIDANTIXINGSHICUNZAIYUBAOJIANGZHONG,CHANSHENGLVSEYINGGUANG(Ex=490 nm, Em=525 nm)。JC-10BUJINKEYONGYUDINGXINGJIANCE,YINYANSEDEBIANHUAKEYIFEICHANGZHIJIEDEFANYINGCHUXIANLITIMODIANWEIDEBIANHUA。YEKEYIYONGYUDINGLIANGJIANCE,YINXIANLITIDEQUJIHUACHENGDUKEYITONGGUOHONG/LVYINGGUANGQIANGDUDEBILILAIHENGLIANG。ZHEILIANGZHONGYANSEDEBIANHUAKEYIYONGLIUSHIXIBAOYISHANGDEBIAOZHUNLVGUANGQIJIANCEDAO,LVSEYINGGUANGKEYONGFL1TONGDAOFENXI,HONGSEYINGGUANGKEYONGFL2TONGDAOFENXI。CHULEYONGYULIUSHIXIBAOSHU,YEKEYIYONGYUYINGGUANGCHENGXIANGHEYINGGUANGMEIBIAOBANJIANCEPINGTAI。

BENSHIJIHEZHONGTIGONGLEYANGXINGDUIZHAOCCCP,QINENGYOUDAOXIANLITIMODIANWEIXIAJIANG。DUIYULIUKONGBANZHONGDEYANGPIN,BENSHIJIHEGONGKEYIJIANCE100GEYANGPIN。

 运输与保存方法

BINGDAI(wet ice)YUNSHU。-20℃GANZAOBIGUANGBAOCUN,FENZHUANG,YIBIMIANFANFUDONGRONG,YINIANYOUXIAO。CHAOCHUNSHUIHEJC-10RANSEHUANCHONGYE(5×)YEKE4℃BAOCUN。

使用方法

上海快31. JC-10RANSEGONGZUOYEDEPEIZHI

上海快3 QUSHILIANGJC-10(200×),ANZHAOMEI50 μL JC-10(200×)JIARU8 mLCHAOCHUNSHUIDEBILIXISHI,JULIEZHENDANGCHONGFENRONGJIEBINGHUNYUNJC-10,RANHOUZAIJIARU2 mL JC-10RANSEHUANCHONGYE(5×),HUNYUNHOUJIWEIJC-10RANSEGONGZUOYE。

2. YANGXINGDUIZHAODESHEZHI

QUCHUSHIJIHEZHONGGONGDECCCP(10 mM)ANZHAO1∶1000DEBILIJIARUDAOXIBAOPEIYANGYEZHONG,SHIQIZHONGNONGDUWEI10 μM,CHULIXIBAO20FENZHONG。SUIHOUANZHAOXIASHUFANGFAZHUANGZAIJC-10,JINXINGXIANLITIMODIANWEIDEJIANCE。DUIYUDADUOSHUXIBAO,TONGCHANG10 μM CCCPCHULI20FENZHONGHOUXIANLITIDEMODIANWEIHUIWANQUANSANGSHI,JC-10RANSEHOUGUANCHAYINGCHENGLVSEYINGGUANG;ERZHENGCHANGDEXIBAOJINGJC-10RANSEHOUYINGXIANSHIHONGSEYINGGUANG。DUIYUTEDINGDEXIBAO,CCCPDEZUOYONGNONGDUHEZUOYONGSHIJIANKENENGYOUSUOBUTONG,XUZIXINGCANKAOXIANGGUANWENXIANZILIAOJUEDING。

上海快33. XIBAORANSE

3.1DUIYUXUANFUXIBAO

上海快31)QU1×105~6×105GEXIBAOZHONGXUANYU0.5 mLXIBAOPEIYANGYEZHONG(KEYIHANXUEQINGHEFENHONG),JIARU0.5 mL JC-10RANSEGONGZUOYE,DIANDAOHUNYUN。

上海快32)37℃FUYU20FENZHONG。FUYUQIJIAN,ANMEI1 mL JC-10RANSEHUANCHONGYE(5×)JIARU4 mLZHENGLIUSHUIDEBILIPEIZHISHILIANGDEJC-10RANSEHUANCHONGYE(1×),BINGZHIYUBINGYU,ZUOWEIXIYE。

上海快33)37℃FUYUJIESHUHOU,600g  4℃LIXIN3~4FENZHONG,QISHANGQING,ZHUYIJINLIANGBUYAOXICHUXIBAO。JIARU1 mL JC-10RANSEHUANCHONGYE(1×)ZHONGXUANXIBAO,600 g, 4℃LIXIN3~4FENZHONG,QISHANGQING,ZHONGFUYICI。

4)YONGSHILIANGJC-10RANSEHUANCHONGYE(1×)ZHONGXUANHOU,YONGYINGGUANGXIANWEIJINGHUOJIGUANGGONGJUJIAOXIANWEIJINGGUANCHA,YEKEYIYONGYINGGUANGFENGUANGGUANGDUJIJIANCEHUOLIUSHIXIBAOYIFENXI。

3.2DUIYUTIEBIXIBAO

1)DUIYU6KONGBAN,XICHUPEIYANGYE,GENJUJUTISHIYANRUYOUBIYAOKEYONGPBSHUOQITASHIDANGRONGYEXIDIXIBAOYICI,JIARU1 mLXIBAOPEIYANGYE(KE

YIHANXUEQINGHEFENHONG)。

2)RANHOUJIARU1 mL JC-10RANSEGONGZUOYE,CHONGFENHUNYUN。XIBAOPEIYANGXIANGZHONG37℃FUYU20FENZHONG。FUYUQIJIAN,ANMEI1 mL JC-10RANSEHUANCHONGYE(5×)JIARU4 mLZHENGLIUSHUIDEBILIPEIZHISHILIANGDEJC-10RANSEHUANCHONGYE(1×),BINGFANGZHIYUBINGYU,ZUOWEIXIYE。

3)37℃FUYUJIESHUHOU,XICHUSHANGQING,YONGJC-10RANSEHUANCHONGYE(1×)XIDI2CI。

4)JIARU2 mLXIBAOPEIYANGYE,PEIYANGYEZHONGKEYIHANYOUXUEQINGHEFENHONG。ZAIYINGGUANGXIANWEIJINGHUOJIGUANGGONGJUJIAOXIANWEIJINGXIAGUANCHA。

【注】:对于贴壁细胞,如果希望采用荧光分光光度计或流式细胞仪检测,可以先收集细胞,重悬后参考悬浮细胞的检测方法。

3.3DUIYUCHUNHUADEXIANLITI

1)BAPEIZHIHAODEJC-10RANSEGONGZUOYEYONGJC-10RANSEHUANCHONGYE(1×)XISHI5BEI。QU5BEIXISHIDEJC-10RANSEGONGZUOYE0.9 mL JIARU0.1 mLZONGDANBAILIANGWEI10~100 μgCHUNHUADEXIANLITI。

上海快32)YONGYINGGUANGFENGUANGGUANGDUJIHUOYINGGUANGMEIBIAOYIJIANCE:HUNYUNHOUZHIJIEYONGYINGGUANGFENGUANGGUANGDUJIJINXINGSHIJIANSAOMIAO(time scan),JIFABOZHANGWEI485 nm,FASHEBOZHANGWEI590 nm。RUGUOSHIYONGYINGGUANGMEIBIAOYI,JIFABOZHANGBUNENGSHEZHIWEI485nmSHI,KEYIZAI475~520nmFANWEINEISHEZHIJIFABOZHANG。

上海快34. YINGGUANGGUANCE

JIANCEJC-10DANTISHIKEYIBAJIFAGUANGSHEZHIWEI490 nm,FASHEGUANGSHEZHIWEI530 nm;JIANCEJC-10JUHEWUSHI,KEYIBAJIFAGUANGSHEZHIWEI525 nm,FASHEGUANGSHEZHIWEI590 nm。

【注】:此处测定荧光时不必把激发光和发射光设置在zui大激发波长和zui大发射波长。如使用荧光显微镜观察,检测JC-10单体时可以参考观察其它绿色荧光时的设置,如观察GFP或FITC时的设置;检测JC-10聚合物时可以参考观察其它红色荧光,如碘化丙啶或Cy3时的设置。

注意事项

1)JC-10(200×)ZAI4℃、BINGYUDENGJIAODIWENDUQINGKUANGXIAHUININGGUERZHANZAILIXINGUANGUANDI、GUANBIHUOGUANGAINEI,KEYI20~25℃SHUIYUWENYUPIANKEZHIQUANBURONGJIEHOUSHIYONG。

上海快32)XUBAJC-10(200×)YONGSHIJIHETIGONGDECHAOCHUNSHUICHONGFENRONGJIEHUNYUNHOUCAIKEJIARUJC-10RANSEHUANCHONGYE(5×)。BUKEXIANPEIZHIJC-10RANSEHUANCHONGYE(1×)ZAIJIARUJC-10(200×),FOUZEJC-10JIANGHENNANCHONGFENRONGJIEBINGHUIYANZHONGYINGXIANGHOUXUDEJIANCESHIYONG。

3)SHIJC-10RANSEHUANCHONGYE(1×)BAOCHI4℃ZUOYOU,CISHIDEXIDIXIAOGUOJIAOHAO。

4)JC-10TANZHENZHUANGZAIWANBINGXIDIHOUJINLIANGZAI30FENZHONGNEIWANCHENGHOUXUJIANCEGUOCHENG。ZAIJIANCEQIANXUBINGYUBAOCUN。

5)QINGWUBAJC-10RANSEHUANCHONGYE(5×)QUANBUPEIZHICHENGJC-10RANSEHUANCHONGYE(1×),BENSHIJIHESHIYONGGUOCHENGZHONGXUZHIJIESHIYONGJC-10RANSEHUANCHONGYE(5×)。

上海快36)RUGUOFAXIANJC-10RANSEHUANCHONGYE(5×)ZHONGYOUCHENDIAN,BIXUQUANBURONGJIEHOUCAINENGSHIYONG,WEICUJINRONGJIEKEYIZAI37℃JIARE。

7)CCCPWEIXIANLITIDIANZICHUANDILIANYIZHIJI,YOUDU,QINGZHUYIXIAOXINFANGHU。

8)WEILENINDEanQUANHEJIANKANG,QINGCHUANSHIYANFUBINGDAIYICIXINGSHOUTAOCAOZUO。

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