上海快3

设为上海快3 | 加入收藏
关于我们上海快3 > 产品中心 > 细胞生物学 > 细胞增殖与凋亡 > FS1157CCK-8细胞增殖及毒性检测试剂
  • CCK-8细胞增殖及毒性检测试剂
  • 型号:FS1157
  • 报价:4100
  • 地区:上海市
  • 0
  • Cell Counting Kit (CCK-8) CCK-8细胞增殖及毒性检测试剂.MTT 噻唑兰;CCK-8;WST-1;EdU 5-乙炔基-2’-脱氧尿苷.
  • 025-58690567
产品详细介绍

Cell Counting Kit (CCK-8) CCK-8细胞增殖及毒性检测试剂

产品信息

产品货号产品名称规格价格(元)
FS1157-1MLCell Counting Kit (CCK-8) CCK-8细胞增殖及毒性检测试剂1ml (100T)150.00 
FS1157-5MLCell Counting Kit (CCK-8) 5ml (500T)500.00 
FS1157-10MLCell Counting Kit (CCK-8) 10ml (1000T)900.00 
FS1157-50MLCell Counting Kit (CCK-8) 50ml (5×1000T)4100.00 
FS1157-100MLCell Counting Kit (CCK-8) 100ml (10×1000T)6480.00 

 

 

产品原理

上海快3Cell Counting Kit-8 (CCK-8) XIBAOHUOXINGJIANCESHIJIHESHIYIZHONGGUANGFANYINGYONGYUXIBAOZENGZHIHEXIBAODUXINGXIANGGUANSHIYANDEKUAISU、GAOLINGMINDUJIANCESHIJIHE。

CCK-8 DEZHUYAOCHENGFENWEISHUIRONGXINGSIZUOYAN[Water Soluble Tetrazolium-8] (2-(2-JIAYANGJI-4-XIAOBENJI)-3-(4-XIAOBEN

JI)-5-(2,4-ERHUANGJIBEN)-2H-SIZUODANNAYAN),Water Soluble Tetrazolium-8 SHIYIZHONGLEISIYUMTT DEHUAHEWU,ZAIDIANZIOUHESHIJICUNZAIDEQINGKUANGXIA,KEYIBEIXIANLITINEIDEYIXIETUOQINGMEIHAIYUANSHENGCHENGCHENGHUANGSEDESHUIRONGXINGDEJIAZA(Formazan,CANKAOTU1)。

Water Soluble Tetrazolium-8 BEIXIBAONEITUOQINGMEISHENGWUHAIYUANHOUSHENGCHENGDEJIAZANENGGOUZHIJIERONGJIEZAIPEIYANGJIZHONG。HUOXIBAODESHUMUYUEDUO,ZEYANSEYUESHEN。DUIYUTONGYANGDEXIBAO,YANSEDESHENQIANHEXIBAOSHUMUCHENGXIANXINGGUANXI。

产品优点

上海快3BENSHIJIHETIGONGLEYIZHONGLINGMINDUGAO、CAOZUOJIANBIAN、SHIYONGANQUANDEXIBAOZENGZHIYUHUOXINGJIANCEFANGFA。YUCHUANTONGDEMTT FAXIANGBIJUYOUMINGXIANDEYOUSHI:

1. MTT FASHENGCHENGDEJIAZABUSHISHUIRONGXINGDE,XUYAOSHIYONG DMSO DENGYOUJIRONGJIRONGJIE;ER CCK-8 CHANSHENGDEJIAZASHISHUIRONGXINGDE,SHENGQULERONGJIEBUZHOU,YINCIJIANSHAOLEGAICAOZUOBUZHOUDAILAIDESHIYANWUCHA。

2. YOUYUCCK-8 CHANSHENGDEJIAZASHISHUIRONGXINGDE,WUXUXIDIAOPEIYANGJIHOU,ZAIYONGDMSO DENGYOUJIRONGJIRONGJIE,FEICHANGSHIHEYONGYUXUANFUXIBAODEHUOXINGCEDING。

上海快33. YUMTT  FANGFAXIANGBI,CCK-8 FAXIANXINGFANWEIGENGKUAN,LINGMINDUGENGGAO。

上海快34. CCK-8 FADUIXIBAOWUDUXING,KEYIDUOCICEDING,XUANQUzuiJIACEDINGSHIJIAN。 

上海快35. CCK-8 FAWUXUPEIZHI,JIKAIJIYONG。

6. CCK-8 FASHIHEDAGUIMO、GAOTONGLIANGDEYANGPINJIANCE。

使用方法

1. ZAI96 KONGBANZHONGPEIZHI100 μL DEXIBAOXUANYE。JIANGPEIYANGBANZAIPEIYANGXIANGYUPEIYANG24 XIAOSHI(ZAI37℃,5% CO2 DETIAOJIANXIA)。

2. XIANGPEIYANGBANJIARU10 μL DEDAICEyaoWUJINXINGCHULI。

3. GENJUSHIYANXUYAO,JIANGPEIYANGBANZAIPEIYANGXIANGFUYUYIDUANSHIJIAN(LIRU:6, 12,24 HUO48 XIAOSHI)。

注:如果不进行yao物处理而直接测定细胞的活性,则不需要2-3 步骤,直接从第4 步操作。

4. XIANGMEIKONGJIARU10 μL CCK-8 RONGYE(ZHUYIBUYAOZAIKONGZHONGSHENGCHENGQIPAO,TAMENHUIYINGXIANGOD ZHIDEDUSHU)。 

5. JIANGPEIYANGBANZAIPEIYANGXIANGNEIFUYU2-4 XIAOSHI。

6. YONGMEIBIAOYICEDINGZAI450 nm CHUDEXIGUANGDU。

上海快37. RUGUOZANSHIBUCEDINGOD ZHI,KEYIXIANGMEIKONGZHONGJIARU10 μL 1% SDS(w/v)RONGYEHUOZHE0.1 M HCl RONGYE,BINGGAISHANGPEIYANGBANGAIZI,ZAISHIWENTIAOJIANXIABIGUANGBAOCUN。24 XIAOSHINEIXIGUANGDUBUHUIFASHENGBIANHUA。

注意事项 

1. YOUYU96 KONGBANZHOUWEIYIQUANzuiRONGYIZHENGFA,ZHOUWEIYIQUANJIANYIBUYONG,GAIJIAPBS HUOSHUI。

2. CCK-8 JIANCEXIBAOHUOXINGDEYUANLISHITONGGUOJIANCEHUOXIBAOTUOQINGMEICUIHUADEFANYING。RUGUODAICETIXIZHONGCUNZAIHAIYUANJI(LIRUYIXIEKANGYANGHUAJI),KENENGHUIGANRAOJIANCEJIEGUO,XUSHEFAQUCHUQIYINGXIANG。 

3. DANGSHIYONGBIAOZHUN96 KONGBANSHI,TIEBIXIBAODEzuiXIAOJIEZHONGLIANGZHISHAOWEI1,000 GE/KONG(100 μL PEIYANGJI)。JIANCEBAIXIBAOSHIDELINGMINDUXIANGDUIJIAODI,YINCITUIJIANJIEZHONGLIANGBUDIYU2,500 GE/KONG(100 μL PEIYANGJI),QIEJIARUCCK-8 HOUFUYUDESHIJIANZHANGYIXIE。 

4. RUGUOXIBAOPEIYANGSHIJIANJIAOZHANG,PEIYANGJIYANSEFASHENGBIANHUA,YINGXIDIXIBAOGENGHUANPEIYANGJIHOUZAIJIACCK-8  JIANCE。 

5. FENHONGHEXUEQINGDUIBENSHIJIHEDECEDINGWUMINGXIANYINGXIANG。PEIYANGJIZHONGFENHONGDEXIGUANGDUKEYIZAIJISUANSHI,TONGGUOKOUCHUZHIHANYOUPEIYANGJIDEDUIZHAOKONGZHONGBENDIDEXIGUANGDUERXIAOCHU,YINCIBUHUIDUIJIANCEJIEGUOZAOCHENGYINGXIANG。

储存条件:上海快3本试剂盒在在-20℃至少可以保存 3 年,4℃只能保存数月。建议收到产品后,放-20℃冰箱冷冻保存。首次使用时,室温融化后,建议按自己实验需要,分装到mie菌离心管(比如 1 mL/管),继续放-20℃冰箱冷冻保存。每次实验时,按需从-20℃冰箱取出相应数量的试剂,室温融化后使用。

BENCHANPINJINGCESHI:5 CIZUOYOUDEFANFUDONGRONG,DUICHANPINZHILIANGJIHUMEIYOURENHEYINGXIANG。RUGUO4℃FANGZHIDESHIJIANCHAOGUOBANNIAN,DUISHIYANDEYINGXIANGXIANGDUIJIAODA。

 

在线留言

留言框

  • 产品:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

  • 省份:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
相关产品
移动电话
15380906406